解剖学和形态学
麻醉学
听力与言语-语言病理学
行为科学
心脏和心血管系统
细胞和组织工程学
临床神经病学
危重症监护医学
牙科,口腔外科和医学
皮肤病学
急诊医学
内分泌学和新陈代谢
肠胃学和肝脏学
老人病学和老年医学
卫生保健科学和服务
血液学
免疫学
传染病
综合和补充性医学
医学伦理学
医学信息学
医学实验室技术
医学,全科和内科
医学,法律
医学,研究和试验
神经系统科学
护理
营养学和饮食学
产科医学和妇科医学
肿瘤学
眼科学
整形外科学
耳鼻喉科学
病理学
儿科学
周围血管疾病
药理学和药剂学
生理学
基本医疗保健
精神病学
公共、环境和职业卫生
放射学,核医学和医学成像
康复学
生殖生物学
呼吸系统
风湿病学
运动科学
外科学
毒理学
热带医学
泌尿学和肾脏学
病毒学
老年医学
健康政策和服务
心理学,临床
abstract::Feedback inhibitory motifs are thought to be important for pattern separation across species. How feedback circuits may implement pattern separation of biologically plausible, temporally structured input in mammals is, however, poorly understood. We have quantitatively determined key properties of netfeedback inhibiti...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.53148
更新日期:2020-02-20 00:00:00
abstract::Perturbation of neuronal activity is key to understanding the brain's functional properties, however, intervention studies typically perturb neurons in a nonspecific manner. Recent optogenetics techniques have enabled patterned perturbations, in which specific patterns of activity can be invoked in identified target n...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.52757
更新日期:2020-02-19 00:00:00
abstract::Khoomei is a unique singing style originating from the republic of Tuva in central Asia. Singers produce two pitches simultaneously: a booming low-frequency rumble alongside a hovering high-pitched whistle-like tone. The biomechanics of this biphonation are not well-understood. Here, we use sound analysis, dynamic mag...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.50476
更新日期:2020-02-17 00:00:00
abstract::Atopic Dermatitis (AD) is a T cell-mediated chronic skin disease and is associated with altered skin barrier integrity. Infants with mutations in genes involved in tissue barrier fitness are predisposed towards inflammatory diseases, but most do not develop or sustain the diseases, suggesting that there exist regulato...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.51188
更新日期:2020-02-17 00:00:00
abstract::Though neurotransmitters are essential elements in neuronal signal transduction, techniques for in vivo analysis are still limited. Here, we describe an organic electrochemical transistor array (OECT-array) technique for monitoring catecholamine neurotransmitters (CA-NTs) in rat brains. The OECT-array is an active sen...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.50345
更新日期:2020-02-11 00:00:00
abstract::The production of action sequences is a fundamental aspect of motor skills. To examine whether primary motor cortex (M1) is involved in maintenance of sequential movements, we trained two monkeys (Cebus apella) to perform two sequential reaching tasks. In one task, sequential movements were instructed by visual cues, ...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.53038
更新日期:2020-02-10 00:00:00
abstract::Whether neurons encode information through their spike rates, their activity times or both is an ongoing debate in systems neuroscience. Here, we tested whether humans can discriminate between a pair of temporal odor mixtures (TOMs) composed of the same two components delivered in rapid succession in either one tempor...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.49734
更新日期:2020-02-07 00:00:00
abstract::Correct neuronal development requires tailored neurite outgrowth. Neurite outgrowth is driven in part by microtubule-sliding - the transport of microtubules along each other. We have recently demonstrated that a 'mitotic' kinesin-6 (Pavarotti in Drosophila) effectively inhibits microtubule-sliding and neurite outgrowt...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.52009
更新日期:2020-02-05 00:00:00
abstract::Tuberculosis disproportionately affects the Canadian Inuit. To address this, it is imperative we understand transmission dynamics in this population. We investigate whether 'deep' sequencing can provide additional resolution compared to standard sequencing, using a well-characterized outbreak from the Arctic (2011-201...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.53245
更新日期:2020-02-04 00:00:00
abstract::The vertebral column or spine assembles around the notochord rod which contains a core made of large vacuolated cells. Each vacuolated cell possesses a single fluid-filled vacuole, and loss or fragmentation of these vacuoles in zebrafish leads to spine kinking. Here, we identified a mutation in the kinase gene dstyk tha...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.51221
更新日期:2020-01-29 00:00:00
abstract::The inferior colliculus (IC) is the major midbrain auditory integration center, where virtually all ascending auditory inputs converge. Although the IC has been extensively studied for sound processing, little is known about the neural activity of the IC in moving subjects, as frequently happens in natural hearing con...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.52228
更新日期:2020-01-28 00:00:00
abstract::Genome-wide association analyses have uncovered multiple genomic regions associated with T2D, but identification of the causal variants at these remains a challenge. There is growing interest in the potential of deep learning models - which predict epigenome features from DNA sequence - to support inference concerning...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.51503
更新日期:2020-01-27 00:00:00
abstract::Bacillus subtilis can measure the activity of the enzymes that remodel the cell wall to ensure that the levels of activity are 'just right'. ...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.54244
更新日期:2020-01-24 00:00:00
abstract::Proprioception, the sense of body position, movement, and associated forces, remains poorly understood, despite its critical role in movement. Most studies of area 2, a proprioceptive area of somatosensory cortex, have simply compared neurons' activities to the movement of the hand through space. Using motion tracking...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.48198
更新日期:2020-01-23 00:00:00
abstract::Studies of cell fate focus on specification, but little is known about maintenance of the differentiated state. In this study, we find that the mouse tendon cell fate requires continuous maintenance in vivo and identify an essential role for TGFβ signaling in maintenance of the tendon cell fate. To examine the role of...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.52695
更新日期:2020-01-21 00:00:00
abstract::Genomic rearrangements associated with speciation often result in variation in chromosome number among closely related species. Malassezia species show variable karyotypes ranging between six and nine chromosomes. Here, we experimentally identified all eight centromeres in M. sympodialis as 3-5-kb long kinetochore-bou...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.53944
更新日期:2020-01-20 00:00:00
abstract::Negative regulators of adult neurogenesis are of particular interest as targets to enhance neuronal repair, but few have yet been identified. Planarians can regenerate their entire CNS using pluripotent adult stem cells, and this process is robustly regulated to ensure that new neurons are produced in proper abundance...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.47293
更新日期:2020-01-20 00:00:00
abstract::Macrophages are highly plastic cells with critical roles in immunity, cancer, and tissue homeostasis, but how these distinct cellular fates are triggered by environmental cues is poorly understood. To uncover how primary murine macrophages respond to bacterial pathogens, we globally assessed changes in post-translatio...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.51461
更新日期:2020-01-17 00:00:00
abstract::The precise pattern of motor neuron (MN) activation is essential for the execution of motor actions; however, the molecular mechanisms that give rise to specific patterns of MN activity are largely unknown. Phrenic MNs integrate multiple inputs to mediate inspiratory activity during breathing and are constrained to fi...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.52859
更新日期:2020-01-16 00:00:00
abstract::SynGAP is a postsynaptic density (PSD) protein that binds to PDZ domains of the scaffold protein PSD-95. We previously reported that heterozygous deletion of Syngap1 in mice is correlated with increased steady-state levels of other key PSD proteins that bind PSD-95, although the level of PSD-95 remains constant (Walku...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.52656
更新日期:2020-01-15 00:00:00
abstract::Microtubules are nucleated from specific locations at precise times in the cell cycle. However, the factors that constitute these microtubule nucleation pathways and their mode of action still need to be identified. Using purified Xenopus laevis proteins we biochemically reconstitute branching microtubule nucleation, ...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.49797
更新日期:2020-01-14 00:00:00
abstract::Intermittent hypoxia (IH) is the predominant pathophysiological disturbance in obstructive sleep apnea (OSA), known to be independently associated with cardiovascular diseases. However, the effect of IH on cardiac fibrosis and molecular events involved in this process are unclear. Here, we tested IH in angiotensin II ...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.49923
更新日期:2020-01-14 00:00:00
abstract::The numerous neurons and glia that form the brain originate from tightly controlled growth and division of neural stem cells, regulated systemically by important known stem cell-extrinsic signals. However, the cell-intrinsic mechanisms that control the distinctive proliferation rates of individual neural stem cells ar...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.51529
更新日期:2020-01-14 00:00:00
abstract::Cytomegaloviruses (CMVs) are ubiquitous pathogens known to employ numerous immunoevasive strategies that significantly impair the ability of the immune system to eliminate the infected cells. Here, we report that the single mouse CMV (MCMV) protein, m154, downregulates multiple surface molecules involved in the activa...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.50803
更新日期:2020-01-13 00:00:00
abstract::The basolateral amygdala (BLA) plays a vital role in associating sensory stimuli with salient valence information. Excitatory principal neurons (PNs) undergo plastic changes to encode this association; however, local BLA inhibitory interneurons (INs) gate PN plasticity via feedforward inhibition (FFI). Despite literat...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.50601
更新日期:2020-01-09 00:00:00
abstract::Key to CRISPR-Cas adaptive immunity is maintaining an ongoing record of invading nucleic acids, a process carried out by the Cas1-Cas2 complex that integrates short segments of foreign genetic material (spacers) into the CRISPR locus. It is hypothesized that Cas1 evolved from casposases, a novel class of transposases....
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.50004
更新日期:2020-01-08 00:00:00
abstract::Pancreatic ATP-sensitive K+ channels (KATP) comprise four inward rectifier subunits (Kir6.2), each associated with a sulphonylurea receptor (SUR1). ATP/ADP binding to Kir6.2 shuts KATP. Mg-nucleotide binding to SUR1 stimulates KATP. In the absence of Mg2+, SUR1 increases the apparent affinity for nucleotide inhibition...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.52775
更新日期:2020-01-07 00:00:00
abstract::Heterogeneity broadly exists in various cell types both during development and at homeostasis. Investigating heterogeneity is crucial for comprehensively understanding the complexity of ontogeny, dynamics, and function of specific cell types. Traditional bulk-labeling techniques are incompetent to dissect heterogeneit...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.52024
更新日期:2020-01-06 00:00:00
abstract::Direct activation of the human phospholipase C-γ isozymes (PLC-γ1, -γ2) by tyrosine phosphorylation is fundamental to the control of diverse biological processes, including chemotaxis, platelet aggregation, and adaptive immunity. In turn, aberrant activation of PLC-γ1 and PLC-γ2 is implicated in inflammation, autoimmu...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.51700
更新日期:2019-12-31 00:00:00
abstract::Eukaryotic DNA is packaged into nucleosome arrays, which are repositioned by chromatin remodeling complexes to control DNA accessibility. The Saccharomyces cerevisiae RSC (Remodeling the Structure of Chromatin) complex, a member of the SWI/SNF chromatin remodeler family, plays critical roles in genome maintenance, tra...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.54449
更新日期:2019-12-30 00:00:00
abstract::Human-based modelling and simulations are becoming ubiquitous in biomedical science due to their ability to augment experimental and clinical investigations. Cardiac electrophysiology is one of the most advanced areas, with cardiac modelling and simulation being considered for virtual testing of pharmacological therap...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.48890
更新日期:2019-12-24 00:00:00
abstract::Lymph nodes (LNs) are a common site of metastasis in solid cancers, and cutaneous melanomas show inherent properties of LN colonization. However, interactions between LN stroma and pioneer metastatic cells during metastatic colonization remain largely uncharacterized. Here we studied mice implanted with GFP-expressing...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.48916
更新日期:2019-12-24 00:00:00
abstract::Anterograde transport of late endosomes or lysosomes (LE/Lys) is crucial for proper axon growth. However, the role of energetic nutrients has been poorly explored. Malonyl-CoA is a precursor of fatty acids, and its intracellular levels highly fluctuate depending on glucose availability or the energy sensor AMP-activat...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.51063
更新日期:2019-12-23 00:00:00
abstract::The contribution of insular cortex to speech production remains unclear and controversial given diverse findings from functional neuroimaging and lesional data. To create a precise spatiotemporal map of insular activity, we performed a series of experiments: single-word articulations of varying complexity, non-speech ...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.53086
更新日期:2019-12-19 00:00:00
abstract::Actin dynamics generate forces to deform the membrane and overcome the cell's high turgor pressure during clathrin-mediated endocytosis (CME) in yeast, but precise molecular details are still unresolved. Our previous models predicted that actin filaments of the endocytic meshwork continually polymerize and disassemble...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.52355
更新日期:2019-12-19 00:00:00
abstract::Human disease agents exist within complex environments that have underappreciated effects on transmission, especially for parasites with multi-host life cycles. We examined the impact of multiple host and parasite species on transmission of the human parasite Schistosoma mansoni in Kenya. We show S. mansoni is impacte...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.50095
更新日期:2019-12-17 00:00:00
abstract::Ribosomes are universally important in biology and their production is dysregulated by developmental disorders, cancer, and virus infection. Although presumed required for protein synthesis, how ribosome biogenesis impacts virus reproduction and cell-intrinsic immune responses remains untested. Surprisingly, we find t...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.49551
更新日期:2019-12-16 00:00:00
abstract::Cardiac conduction defects decrease life expectancy in myotonic dystrophy type 1 (DM1), a CTG repeat disorder involving misbalance between two RNA binding factors, MBNL1 and CELF1. However, how DM1 condition translates into conduction disorders remains poorly understood. Here we simulated MBNL1 and CELF1 misbalance in...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.51114
更新日期:2019-12-12 00:00:00
abstract::Mammalian two-pore-channels (TPC1, 2; TPCN1, TPCN2) are ubiquitously- expressed, PI(3,5)P2-activated, Na+-selective channels in the endosomes and lysosomes that regulate luminal pH homeostasis, membrane trafficking, and Ebola viral infection. Whereas the channel activity of TPC1 is strongly dependent on membrane volta...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.51423
更新日期:2019-12-11 00:00:00
abstract::Recent transcriptional profiling technologies are uncovering previously-undefined cell populations and molecular markers at an unprecedented pace. While single cell RNA (scRNA) sequencing is an attractive approach for unbiased transcriptional profiling of all cell types, a complementary method to isolate and sequence ...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.51452
更新日期:2019-12-09 00:00:00